14. February 2021
Broccoli - infer with high precision orthologous groups and pairs of proteins using a mixed phylogeny-network approach
https://github.com/rderelle/Broccoli
dependency
DIAMOND
Fasttree
manual
https://github.com/rderelle/Broccoli/blob/master/manual_Broccoli_v1.2.pdf
usage on sapelo2
1#!/bin/sh
2#SBATCH -J broccoli
3#SBATCH --partition
4#SBATCH --nodes=1
5#SBATCH --ntasks-per-node=32
6#SBATCH --time=120:00:00
7#SBATCH --mem=480gb
8#SBATCH --mail-type=BEGIN,END,FAIL
9#SBATCH --output=.%j.out
10#SBATCH --error=.%j.err
11
12
13module load FastTree/2.1.11-GCCcore-8.3.0
14
15ml Anaconda3
16
17ml DIAMOND
18conda init bash
19source ~/.bashrc
20conda activate binf
21
22cd $SLURM_SUBMIT_DIR
23
24python broccoli.py -dir /fastaFiles -threads 32 -ext '.fa' -path_fasttree /apps/eb/FastTree/2.1.11-GCCcore-8.3.0/bin/FastTree
SEFF
Cores per node: 32
CPU Utilized: 12-08:29:52
CPU Efficiency: 91.35% of 13-12:35:12 core-walltime
Memory Utilized: 229.27 GB
Memory Efficiency: 47.76% of 480.00 GB